Description
Aviva Systems Biology 5-Me-THF ELISA Kit (OKCD02251) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-5-Me-THF antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 5-Me-THF and incubated. The 5-Me-THF found in the sample or standards competes with the biotinylated 5-Me-THF for limited binding sites on the immobilized anti-5-Me-THF antibody. Wells are washed and Avidin-HRP conjugate is added, incubated then washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 5-Me-THF captured in the well and inversely proportional to the amount of 5-Me-THF which was contained in the sample or standard